DESCRIPTION:

A fully validated, highly specific rabbit polyclonal antibody to Ryanodine Receptor 2 phospho Serine-2808

The Ryanodine Receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to ryanodine receptor 2 (RyR2) opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. Abnormal structure and function of ryanodine receptors has been reported in failing hearts, with Ser-2808 phosphorylation appearing elevated in clinical situations which may contribute to the abnormal Ca2+ handling characteristics of cardiac muscle in these conditions. Ser-2808 can be phosphorylated in vitro by PKA or CaMKII, which is coincident with significant change in ryanodine receptor 2 RyR2 channel function typified by an increased open probability, the abrogation of the inhibitory effects of CaM, and Mg2+, dissociation of regulatory factors, expression of subconductance states and the expression of channel activity at diastolic Ca2+ concentrations. Serine-2808 in human RyR2 equates to Ser-2809 in rabbit RyR2.

Tested applications:Western blot (1:5000 dilution), IHC Microscopy (1:100 dilution).Species recognition:human, rat, mouse, rabbit, dog, canine and sheep.

DATA SHEET:

Description Lyophilised Rabbit polyclonal serum containing IgG antibody specific to RyR2 Phospho Ser-2808.
Immunogen Synthetic peptide (YNRTRRIS(PO3H2)QT2810) conjugated to keyhole limpet haemocyanin.
Isotype IgG
Purification Raw serum
Species reactivity The antibody recognises phosphorylated Serine-2808 of the ryanodine receptor and binding is blocked in the presence of a peptide containing the Phospho Ser-2808 epitope.
Tested applications WB, IHC, ELISA
Recommended dilution WB 1:5000, IHC 1:100
Optimization Optimal dilutions to be determined by end user
Storage Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles.
Storage Lyophilised: 4 °C Reconstituted:-80 °C
Datasheet A010-30 Lot 1209-03A010-30 Lot 0212-03
MSDS A010-30
Regulatory statement Product for research use only. Not intended for diagnostic or therapeutic use.